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Image Search Results
Journal: Life
Article Title: Accurate Quantitative Histomorphometric-Mathematical Image Analysis Methodology of Rodent Testicular Tissue and Its Possible Future Research Perspectives in Andrology and Reproductive Medicine
doi: 10.3390/life12020189
Figure Lengend Snippet: Representative images of immunohistochemically stained testicular tissue. ( a , b ) Stained against vitamin D receptor protein with Hematoxylin-counterstaining (Control group). ( c , d ) Stained against MAGE-A4, a specific spermatogonium marker protein (Vitamin D deficient group). The images were taken with a Nikon Eclipse Ni-U microscope coupled with a Nikon DS-Ri2 camera using NIS Elements BR and Image J 1.50b image analysis software. The brown color shows DAB chromogen-positivity which indicates receptor-positivity, and the blue/violet color represents hematoxylin counterstaining. ( b , d ) Single tubules from ( a , c ) panels without background, the previously round-selected tubules have been cleared of background with the ‘Image/Clear outside’ tool from the Image J program. 10× objective, scale bars: 200 μm.
Article Snippet: The images were taken with a Nikon Eclipse Ni-U microscope coupled with a
Techniques: Staining, Marker, Microscopy, Software